Infrastructure
MAJOR RESEARCH FACILITIES OF HGRC
The Human Genetics Research Centre (HGRC) is equipped to conduct advanced research in molecular biology, genomics, and translational medicine. The centre supports a wide range of experimental and analytical studies that contribute to understanding the genetic basis of diseases and developing innovative diagnostic and therapeutic approaches.
Human Genetics Research Centre (DST-FIST) Funded facility includes Molecular Genetics Laboratory and Microbial Genetics Laboratory. The Centre is fully equipped to enable all clinicians and student researchers to pursue real-time research.
DST-FIST SPONSORED MOLECULAR GENETICS LABORATORY
DST-FIST SPONSORED MOLECULAR GENETICS LABORATORY
DST-FIST SPONSORED MICROBIAL GENETICS LABORATORY
CORE FACILITIES
The Human Genetics Research Centre (HGRC) Core Facility is a comprehensive, state-of-the-art infrastructure supporting advanced research in molecular biology, genetics, and precision medicine.
Designed to meet both academic and clinical research needs, the facility integrates cutting-edge technologies for accurate experimentation and analysis.
It is equipped with Real-Time PCR Systems for gene amplification, Gene Expression Workstations for functional studies, and DNA/RNA Extraction Platforms for high-quality nucleic acid isolation. Advanced Bioinformatics Workstations and Genomic Data Analysis Tools enable efficient interpretation of complex genetic data. The facility also includes Gel Electrophoresis Systems, Nucleic Acid Quantification Systems, and PCR & Restriction Enzyme Digestion Platforms for routine molecular workflows.
Additionally, Molecular Imaging Systems aid in visualization, while Deep Freezer Storage Facilities ensure secure sample preservation. The HGRC Core Facility empowers researchers with reliable, high-throughput tools, fostering innovation and excellence in genetic research, diagnostics, and personalized healthcare solutions.
MAJOR RESEARCH CAPABILITIES
The Human Genetics Research Centre (HGRC) offers a wide spectrum of advanced research capabilities, supporting innovation in molecular biology, genetics, and biomedical sciences. The centre specializes in gene mutation and genetic polymorphism analysis, enabling the identification of genetic variations associated with diseases. It also conducts mitochondrial functional analysis to study cellular energy mechanisms and related disorders.
HGRC is equipped for both quantitative and qualitative gene expression analysis, providing insights into gene regulation and function. Expertise in Restriction Fragment Length Polymorphism (RFLP) and DNA/RNA extraction, purification, and quantification ensures precise molecular investigations. The facility supports molecular diagnostics and pathogen detection, aiding in early disease identification.
Additionally, the centre performs protein analysis and enzyme kinetics studies, along with agarose and polyacrylamide gel electrophoresis techniques. Its capabilities extend to biomarker discovery, molecular screening, and DNA transformation for gene cloning, fostering cutting-edge research and translational applications.
The facilities and capabilities of HGRC support interdisciplinary research in genetic analysis, biomarker discovery across dentistry, medicine, biotechnology, and biomedical sciences
CONSULTANCY SERVICES
The HGRC provides laboratory services to support faculty research, postgraduate dissertations, and PhD research programs.
| S.No | SERVICES | COST PER SAMPLE (INR) |
| GENOMICS | ||
| 1 | Genomic DNA Isolation (Blood, Tissue, Saliva) | 75 |
| 2 | DNA & RNA Purity and Estimation | 100 |
| 3 | Polymerase Chain Reaction (PCR) | 100 |
| 4 | Genotyping - RFLP | 100 |
| 5 | Restriction Enzyme Cost (Not Included) | 25,000 |
| 6 | Genetic Variant / Mutation Analysis (100 Samples) | - |
| 7 | Sanger Sequencing (SNP Analysis - 10 Samples) | 13,500 |
| 8 | RNA Isolation (Manual) | 100 |
| 9 | cDNA Conversion | 150 |
| 10 | Copy Number Identification (Real-Time PCR) | 200 |
| 11 | Gene Expression Analysis (Real-Time PCR) | 250 |
| 12 | Gene Expression (50 Samples) | 35,000 |
| PROTEOMICS | ||
| 13 | Protein Estimation & Quantification (ELISA) | 20,000 |
| BIOINFORMATICS | ||
| 14 | Genetic Variant / Mutation Analysis | 5,000 |
| 15 | Differential Gene Expression Analysis | 8,000 |
| 16 | Molecular Docking and Simulation | 15,000 |
| SCIENTIFIC REVIEW | ||
| 17 | Meta-analysis & Systematic Review | 10,000 |
| 18 | SNP Analysis | 8,000 |
These services facilitate high-quality experimental research and enhance the translational potential of genetic studies.
HGRC DST-FIST SPONSORED EQUIPMENTS
| S. No | Equipment | Model & Manufacturer Details | Key Applications |
| 1 | Real-Time PCR System | 9001650, Rotor-Gene Q | Gene expression analysis, SNP genotyping, quantitative methylation studies, pathogen detection, and HRM analysis |
| 2 | Nano Fluorometer | E6150, Promega | Molecular biology research, protein analysis, and nucleic acid quality control |
| 3 | UV Spectrophotometer | 4010110100, DLAB Scientific | Nucleic acid quantification, protein analysis, enzyme kinetics, and drug testing |
| 4 | Gradient PCR System | 4375786, Thermo Fisher Scientific | Primer optimization, troubleshooting PCR amplification, and optimizing difficult PCR reactions |
| 5 | Thermo Cooling Centrifuge | C24 PLUS, Remi | Blood component separation, PRP preparation, nucleic acid & protein extraction, microbiological processing |
| 6 | Deep Freezer (-20°C) | BFS345S, Vestfrost | Storage of nucleic acids, enzymes, reagents, microbial cultures, and biobank samples |
| 7 | Laminar UV Hood | VM1200-SS, General Instruments | Sterile and contamination-free workspace for aseptic procedures |
| 8 | Electronic Analytical Balance | AUW220D, Shimadzu | Precise reagent weighing, density determination, piece counting, and moisture analysis |
| 9 | Trinocular Microscope | CX2li-WC, Lawrence & Mayo | Microscopic visualization with digital imaging for research documentation |
| 10 | Laboratory Refrigerator | RLR-400, Remi | Storage of biological samples, reagents, and temperature-sensitive materials |
| 11 | Electroporator | 4309000019, Eppendorf | Introduction of DNA/RNA/proteins into cells for genetic engineering |
| 12 | Gel Documentation System | Gelstain 4X, Mediccare | Visualization and analysis of DNA/RNA and protein gels |
| 13 | Agarose Gel Electrophoresis Unit | EPSV0004, MX Series, Medox | DNA separation, PCR analysis, cloning, restriction mapping, Southern blot prep |
| 14 | Micropipettes | SL-2-1000 Series, Rainin & Plastx | Accurate liquid handling for molecular biology experiments |
| 15 | Laboratory Centrifuge | R-8C BL, Remi | Routine centrifugation for molecular biology and clinical samples |
| 16 | Refrigerated Orbital Incubator | Orbitek-LE (LE-D), Scigenics | Microbial culture, protein expression, enzyme and genetic studies |
| 17 | Microbial Incubator | 7251-150, Equitron | Bacterial culture growth and microbiological research |
| 18 | Ice Flaker | LMIF-30, Labman | Production of sterile cooling ice for lab experiments |
| 19 | ELISA Plate Reader | iMark Microplate Reader, Bio-Rad | Quantitative protein analysis and molecular interaction studies |
HGRC EQUIPMENT
| S. No | Generic Name of Equipment | Model / Make |
| 1 | Spectrophotometer | SL-210, Elico |
| 2 | Mastercycler | Eppendorf |
| 3 | Gel Documentation System with UV Transilluminator | Medicare |
| 4 | Centrifuge R-5148 (Non-Cooling) | Eppendorf |
| 5 | Vortex Mixer | Spinix |
| 6 | Heating Block | Rivotek |
| 7 | Agarose Gel Apparatus | Medox |
| 8 | Vertical Slab Gel Electrophoresis | Medox |
| 9 | Microfuge (Table Top) | Tarsons |
| 10 | pH Meter | Medox |
| 11 | Weighing Balance | Kern |
| 12 | Microwave Oven | Samsung |
| 13 | Pipettes (Adjustable) | Eppendorf & Aqupet |
| 14 | Gel Rocker | Heidolph Titramax 100 |
| 15 | Refrigerator | Godrej |
Ethics and Research Compliance
All research activities are conducted following institutional ethical guidelines and are approved by the Institutional Ethics Committee of Sree Balaji Dental College & Hospital. The centre adheres to national and international standards for responsible biomedical research.